Abstract
Abstract This work is the first report dealing with the identification and characterization of volatile components in hexane and dichloromethane extracts from Buxus sempervirens leaves. Extracts were obtained by acid-base extraction from aqueous mixtures at various pH values (3.5, 6.0 and 9.5) using hexane (for fatty acids, terpenes) and dichloromethane (long chain alcohols, amines, phenols). Our study showed that in many cases these extracts more intensively affect multi-resistant strains of microorganisms than the macrolide antibiotic azithromycin and are therefore a source of molecules to be exploited in medicine or by the pharmaceutical industry. It was shown that the extracts of B. sempervirens can compete with azithromycin by acting on strains of the following microorganisms: Campylobacter jejuni, Staphylococcus aureus, Bacillus subtilis, B. cereus, Listeria іnnocua, L. monocytogenes, Corinebacterium xerosis, Rhodococcus equi and Candida albicans. Using GC–MS we found more than 20 compounds belonging to fatty acid ethers, diterpenes, alcohols, phenols etc.
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