Chemical composition and antibacterial activities of the ethanol extracts from the leaves and tubers of Amorphophallus pusillus

Hong-Thien Van,Hong Hai-My Nguyen,Van-Son Le,Gia-Buu Tran,Nguyen Tuong-An Huynh

doi:10.14719/pst.2020.7.2.732

Abstract

Amorphophallus pusillus is a rare species which is found only in Binh Chau-Phuoc Buu Nature Reserve. In this study, we determined 7 flavonoid compounds in tuber and leaf of A. pusillus, including of vitexin, orientin, vitexin 2?-O-glucoside, cyanidin 3-O-rutinoside, pelargonidin 3-O-glucoside, schaftoside, and peonidin 3-O-rutinoside via liquid chromatography-mass spectrometry (LC-MS). Furthermore, we have proved the antibacterial activities of ethanol extracts obtained from A. pusillus leaves and tubers in the first time. The data revealed that ethanol extracts could inhibit the growth of 6 tested microorganisms, such as Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, and Staphylococcus aureus. These data suggested the potential application of ethanol extracts isolated from this species as natural antimicrobial agents for treatment of infection caused by bacteria, especially in dermatologic and enteric infections.

Highlights

Amorphophallus is a large genus of the Araceae family which comprises of 200 species over the world with 30 species in Vietnam [1,2,3,4]
We have proved the antibacterial activities of ethanol extracts obtained from A. pusillus leaves and tubers for the first time
Based on comparison of mass of compounds (m/z) which were identified in A. pusillus with other compounds in Amorphophallus titanum, other

Summary

Introduction

Amorphophallus is a large genus of the Araceae family which comprises of 200 species over the world with 30 species in Vietnam [1,2,3,4]. Amorphophallus pusillus was described by Hetterschied and Serebryanyi from Xuyen Moc District, Ba Ria-Vung Tau Province, Vietnam [7]. This is an endemic species of Vietnam till date, A. pusillus has only been mentioned. We elucidated the chemical composition via liquid chromatography-mass spectrometry (LC/MS) and proved antibacterial activities of ethanol extracts of A. pusillus leaves and tubers, which provides the information for further application and improves the value of A. pusillus. Chemical composition and antibacterial activities of the ethanol extracts from the leaves and tubers of Amorphophallus pusillus.

Materials and Methods

Extraction procedure

Results and Discussion

Conclusion