Abstract
Prebiotics are non-digestible food ingredients that are selectively fermented by probiotics. The aim of this study was to investigate the chemical properties of a polysaccharide extracted from Cassia fistula mature fruit pulp and to evaluate its effects on probiotic strains: L. casei, L. rhamnosus, E. coli Nissle 1917 (EcN), and E. faecalis. These strains were compared for their growth behavior in culture media supplemented with different Cassia fistula polysaccharide (CFP) concentrations. The molecular weight of CFP was approximately 8.707 × 105 Da. The recovered polysaccharide contained a low percentage of crude protein (4.4%). Aspartic acid, glutamic acid, and proline were the most abundant amino acids. Glucose and mannose were the predominant sugars followed by arabinose and rhamnose. L. casei grew faster at high CFP concentrations (2%) compared with the lower concentrations of CFP. The highest values for the prebiotic index and prebiotic activity score were observed for L. casei treated with 2% CFP, and it may be considered a prebiotic due to its high resistance against α-amylase and acidic conditions. CFP provides two ways to adjust nitric oxide (NO) synthesis in macrophages. Finally, the use of 1.5 and 2% CFP for cultured milk production significantly shortened the fermentation period from 210 min to 180 min and 150 min, respectively.
Highlights
Cassia stula is a medium-sized, fast-growing, deciduous tree native to Srilanka, India, and the Amazon region
The hot-water extract from the dried fruit pulp of C. stula was precipitated with 80% ethanol to give the crude polysaccharides, named Cassia fistula polysaccharide (CFP) which gave 11.25% yield of the dried raw material
This study revealed that a new high molecular weight polysaccharide was extracted from Cassia stula mature fruit pulp for the rst time
Summary
Cassia stula is a medium-sized, fast-growing, deciduous tree native to Srilanka, India, and the Amazon region. It is extensively cultivated worldwide as an ornamental tree for its beautiful yellow owers. Stula.[1,5,6] The aforementioned secondary metabolites are well-recognized as exhibiting various biological activities. In vitro antioxidant activity of hydroalcoholic seed extracts of Cassia stula has been reported.[7] Amento avone isolated from C. Stula have focused on the ripe phase of the fruit pulp and diverse biological properties such as hypolipidemic, anticancer, antioxidant and antibacterial abilities have been stated.[10,11,12] The recent studies on C. stula have focused on the ripe phase of the fruit pulp and diverse biological properties such as hypolipidemic, anticancer, antioxidant and antibacterial abilities have been stated.[10,11,12]
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