Abstract
The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab hepatopancreases, porcine pancreases, and Bacillus subtilis bacteria. The activity of enzymes from crab hepatopancreases was significantly higher than the activity of enzymes derived from other sources. The optimal conditions of the hydrolysis process have been figured out. The samples of cod processing waste hydrolysate were analyzed for amino acid composition and molecular weight distribution. The samples of hydrolysate were used as core components for bacterial culture medium samples. The efficiency of the medium samples was tested for Escherichia coli growth rate; the most efficient sample had an efficiency of 95.3% of that of a commercially available medium based on fish meal. Substitution of medium components with those derived from industrial by-products is one of the ways to decrease a cost of a culture medium in biopharmaceutical drug production.
Highlights
The connective tissue of living organisms plays an important structural and protective role forms a supporting frame and outer covers of all organs
The samples of cod processing waste were hydrolyzed with the EPs; HD was calculated as the ratio of free amino nitrogen (FAN) content to total N content
The activity of the EP derived from red king crab hepatopancreas for the cod processing waste collagen was significantly higher (ANOVA; p < 0.01) than the activity of Pancreatin and Protosubtilin, despite its activity for sodium caseinate was significantly lower than that of Pancreatin
Summary
The connective tissue of living organisms plays an important structural and protective role forms a supporting frame (stroma) and outer covers (dermis) of all organs. The production of fish products is accompanied by a large amount of protein-containing waste, which makes up from 30 to 70% of the feedstock and contains mainly connective tissue proteins including collagen. In this regard, the isolation and use of fish collagen can solve the problem of reducing waste from the marine fishery and fish processing industry. It is reasonable to study the Atlantic cod collagen hydrolyzed using EP derived from red king crab and to estimate its potential as a core component of a bacterial culture medium
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