Chemical characterization, antioxidant, anticholinesterase and alpha-glucosidase potentials of essential oil of Rosmarinus tournefortii de noé

Abstract

The aim of the present work is to characterize the chemical composition of the essential oil and to determinate the antioxidant, anticholinesterase and α-glucosidase activities of Rosmarinus tournefortii de Noe. The essential oil of fresh aerial part of Rosmarinus tournefortii de Noe was analyzed by GC–MS and revealed the presence of 1,8-Cineole (55.26%), l-Borneol (9.57%) and β-Pinene (9.41%) and were identified as major compounds. The antioxidant activity was assessed using different methods, the anticholinesterase activity was determined by AChE and BChE and the α-glucosidase activity was evaluated using 4-Nitrophenyl-α-d-glucopyranoside as substrate. The antioxidant activity showed the good antioxidant activity of the butanolic and chloroform extracts in DPPH, ABTS·+, O2− DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. Furthermore the anticholinesterase activity against acetylcholinesterase of the essential oil exhibited the highest activity, very close to the standard galantamine and for butyrylcholinesterase the chloroform extract exhibited activity higher than the standard galantamine. Moreover the α-glucosidase inhibitory of the chloroform extract showed a higher activity more than the standard acarbose. These results suggest a possible use of Rosmarinus tournefortii de Noe as a potential source of bioactive molecules with anticholinesterase antioxidant and antidiabetic properties