Abstract
BackgroundS. sylvestre Wright is an extremely rare plant, found only in the sub-Himalayan Terai region of West Bengal and neighboring Sikkim foot-hills. The plant has never been evaluated for any pharmaceutical properties. The phytochemical status of the plant is still unknown. Therefore, the aim of the study was to explore the antioxidant and free radical scavenging activities and analysis of bioactive compounds present in S. sylvestre.MethodsS. sylvestre methanolic extract (SSME) was evaluated for different free radical scavenging activities such as hydroxyl radical, nitric oxide, singlet oxygen, hypochlorous acid, peroxynitrite, superoxide radical and hydrogen peroxide scavenging etc. Iron chelating capacity and inhibition of lipid peroxidation were studied in addition to the assessment of haemolytic activity and erythrocyte membrane stabilizing activity (EMSA). Chemical characterization of SSME were performed by high performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC-MS).ResultsThe results indicate that SSME possess potent antioxidant activity with IC50 value of 113.06 ± 5.67 μg/ml, 63.93 ± 4.16 μg/ml and 142.14 ± 6.13 μg/ml for hydroxyl radical, superoxide radical and hypochlorous acid, respectively. HPLC analysis revealed presence of different phenolic secondary metabolites such as gallic acid, ferulic acid, p-coumaric acid, syringic acid, myricetin, quercetin etc. GC-MS analysis displayed the predominance of γ-sitosterol, vitamin E and squalene in SSME.ConclusionThe present study provides a convincing evidence that S. sylvestre not only possess potent antioxidant activity but also can be used as a source of natural bioactive phytochemicals in the future.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-015-0629-0) contains supplementary material, which is available to authorized users.
Highlights
S. sylvestre Wright is an extremely rare plant, found only in the sub-Himalayan Terai region of West Bengal and neighboring Sikkim foot-hills
Chemical characterization were performed by spectrophotometric quantification of total phenolic and flavonoid contents and by chromatographic methods
Antioxidant and free radical scavenging activity Antioxidant and free radical scavenging capacities of sylvestre methanolic extract (SSME) and corresponding standards are demonstrated in Figures 2, 3, 4, and 5
Summary
S. sylvestre Wright is an extremely rare plant, found only in the sub-Himalayan Terai region of West Bengal and neighboring Sikkim foot-hills. The plant has never been evaluated for any pharmaceutical properties. The phytochemical status of the plant is still unknown. The aim of the study was to explore the antioxidant and free radical scavenging activities and analysis of bioactive compounds present in S. sylvestre. The genus Streptocaulon belonging to the family Apocynaceae (previously Asclepiadaceae) having extensive distribution in tropical Asia. Venter and Verhoeven described nine distinct species under the genus Streptocaulon [1]. According to ‘The Plant List’, 23 plants are enlisted under Streptocaulon with 6 accepted, 10 synonyms and 7 unresolved names [2] including S. Chemical characterization were performed by spectrophotometric quantification of total phenolic and flavonoid contents and by chromatographic methods
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
