Chemical Characterization and Antiplatelet Potential of Bioactive Extract from Tomato Pomace (Byproduct of Tomato Paste).

Ivan Palomo,Bárbara Sáez,Anibal Concha-Meyer,Adriana Vásquez,Eduardo Fuentes,Mariane Lutz,Macarena Said

doi:10.3390/nu11020456

Ivan Palomo, Bárbara Sáez + Show 5 more

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https://doi.org/10.3390/nu11020456

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Abstract

We examined the ability of tomato pomace extract (by-product) to affect platelet aggregation in healthy humans (clinical pilot study). In phase 1 the tolerance of participants (n = 15; 5 per dose level) ingesting tomato pomace extract across three dose levels (1, 2.5, and 10 g) was evaluated. Phase 2 was a single-blind, placebo-controlled, parallel design human (male, n = 99; 33 per group) pilot intervention trial investigating the acute and repeated dose effects (5 days) of different doses of tomato pomace extract (1 g, 2.5 g or placebo) on platelet aggregation ex vivo. Various flavonoids (coumaric acid, floridzin, floretin, procyanidin B2, luteolin-7-O-glucoside, kaempferol, and quercitin) and nucleosides (adenosine, inosine, and guanosine) were identified in the tomato pomace extract. The clinical study showed that the daily consumption of 1 g of aqueous extract of tomato pomace for 5 days exerted an inhibitory activity on platelet aggregation.

Highlights

Epidemiological evidence has shown that tomatoes and tomato based products are associated with a reduced risk of cardiovascular diseases (CVD) [1,2]
The aim of this study was to determine the effects of the dietary intake of Tomato pomace (TP) extracts at two different doses on platelet aggregation in adult male healthy subjects
The TP aqueous extract presented a high content of most of the detected compounds, with the exception of gallic and ferulic acids, apigenin-7-O-glucoside, genistein, quercitrin, daidzein, rutin, and epicatechin, which appeared in low concentrations, in some cases very close to the limit of detection

Summary

Introduction

Epidemiological evidence has shown that tomatoes and tomato based products are associated with a reduced risk of cardiovascular diseases (CVD) [1,2]. This association is supported by clinical intervention trials demonstrating the ability of the dietary intake of tomatoes and tomato based products to improve lipid profile [3,4,5], induce anti-inflammatory [5] and antiplatelet activities [6,7]. Modulation of platelet activity is largely achieved through prescribed pharmacological agents such as aspirin and glycoprotein inhibitors, approach used in the primary and secondary prevention of coronary events in high risk individuals [9]. A dietary approach to maintaining cardiovascular health may be considered another important tool in the prevention of CVD [10,11,12]

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