Chemical and organic characteristics of PM2.5 particles and their in-vitro cytotoxic effects on lung cells: The Middle East dust storms in Ahvaz, Iran

Abstract

There are very few reports about the effect of PM2.5 particles carried by the Middle East dust storms on lung cells. This study aimed to investigate the chemical properties and in-vitro cytotoxic effects of PM2.5 extracts. Water-soluble fraction and organic solvent-extractable components of the samples collected from the city of Ahvaz, Khuzestan Province, Iran, during the normal and dust storm days of the winter of 2016 were analyzed and then applied on the human lung epithelial cell line (A549). The chemical properties and the cytotoxicity were analyzed by ICP-OES and Lactase Dehydrogenase (LDH) assay, respectively. The results of the independent t-test showed significantly higher mean cytotoxicity in the samples of normal days than that of dust storm days. It could be due to the fact that the cells were exposed to the same amount of water-soluble extract, whether it be from a normal day or a dust storm day, and since the normal air of Ahvaz is severely affected by traffic and industrial pollutants, its normal day samples showed stronger cytotoxic effect. The results of the Kruskal-Wallis test showed that cytotoxic effect increased with the particulate concentration. The highest cytotoxicity levels observed at 62, 125, and 250 μg/mL concentrations after 24-h incubation were 17% (belonging to a dust storm day), 37% (belonging to a normal day), and 47% (belonging to a dust storm day), respectively Since PAH compounds were measured in the particulate phase, their amount was naturally correlated with the quantity of particulates. However, it was observed that the samples of normal days contained the species with stronger human carcinogens ΣPAHs = 0.82 ng/m3.The results of one-way ANOVA showed a significant difference between the incubation times regarding the resulted cytotoxicity levels. In general, as the incubation duration increased, so did the cytotoxicity level. Although normal day particulates had a greater cytotoxic effect on A549 cell line, dust storm days are associated with higher levels of health risk; simply because during dust storms, people inhale much larger amounts of particulates.