Abstract
Along with an indispensable role in healthcare system of China for centuries, Traditional Chinese Medicine (TCM) shows increasing usages as complementary therapy in western countries. To improve our understanding on their therapeutic effects, it’s critical to unveil chemical compositions of TCM formula, the predominant form of therapy in TCM. However, intrinsic chemical complexity makes it a challenging task to perform analysis on each individual TCM formula even with most current state-of-art analytic techniques available. In this work we approached this question by focusing on analogous formulae, a unique category of TCM formulae grouped together based on shared herbs and/or similar TCM syndromes. Systematic chemical profiling on five Si-Ni decoctions (SNs) for cardiovascular diseases was performed by multistage MS and high-resolution MS (HR-MS) experiments. A total of 83 compounds, including alkaloids, flavonoids, ginsenosides, bile acids and triterpenoids, were described. Analysis on SNs-treated rats detected 55 prototype compounds and 39 metabolites in the systemic circulation in vivo, which may contribute directly to their observed clinical efficacies. This approach offers great advantage to speed up identification of chemical compositions of formula and reveal the difference among these analogous formulae that may be related to diverse clinical effects.
Highlights
Along with an indispensable role in healthcare system of China for centuries, Traditional Chinese Medicine (TCM) shows increasing usages as complementary therapy in western countries
Wang et al studied the chemical constituents in Da-Huang-Gan-Cao-Tang, a famous formula consisting of Rhei Radix et Rhizoma (Da Huang) and Glycyrrhizae Radix et Rhizoma (Gan Cao) in ratio of 4:1 w/w in Jin-Gui-Yao-Lue, using the combination of LC-ESI-Q-TOF-MS and LC-ESI-IT-MS5. 104 compounds were identified on the basis of their accurate molecular weight and multistage MS data
The repeatability and stability of the method were satisfactory with the peak area and retention time variations for nine constituents less than 9.21% and 0.35%, respectively
Summary
Along with an indispensable role in healthcare system of China for centuries, Traditional Chinese Medicine (TCM) shows increasing usages as complementary therapy in western countries. Analysis on SNs-treated rats detected 55 prototype compounds and 39 metabolites in the systemic circulation in vivo, which may contribute directly to their observed clinical efficacies This approach offers great advantage to speed up identification of chemical compositions of formula and reveal the difference among these analogous formulae that may be related to diverse clinical effects. The pre-classification strategy[17] on multiple spectrums of TCM formula was previous proposed by our group on the basis that each class of nature products, such as flavonoids, alkaloids, and saponins, shares a characteristic carbon skeleton or the same structural units It improved the accuracy and sped up the identification of compounds from chemical databases and relevant literatures. Results from this work will facilitate further research in pharmacological basis for clinical application of these five formulae
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