Chemi- and bio-luminescence of coelenterazine analogues. Effect of substituents at the C-2 position

Abstract

Light intensity measurements of recombinant aequorin and semisynthetic aequorins containing coelenterazine analogues with different substituents at the C-2 position showed that a benzyl group in the C-2 position is essential for efficient luminescence activity as revealed by a two-step incubation procedure used to determine why some analogues gave lower luminescence activities than others.