Abstract
Membrane-permeable, phenanthrene-derivedo-quinodimethanes of type 1 react with nitric oxide in a cheletropic fashion to produce fluorescent nitroxide radicals 2 and hydroxylamines 3. This method allows the sensitive and quantitative detection of nitric oxide production in biological samples by means of fluorescence microscopy, as is demonstrated by the monitoring of NO production from alveolar macrophages.
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