Abstract
Abstract Experiments were conducted to test the ability of ferrous iron (Fe2+) chelating agents to discriminate between Fe2+ and ferric iron (Fe3+), to determine ranges of Fe2+ detection in reactions, to determine interactions of chelating agents with phosphorus (P) which is common in plant tissue, and to evaluate some of the complexities of methodology for Fe2+ determinations. PDTS [ferrozine; 3‐(2 pyridyl)‐5,6‐bis (4‐phenyl‐sulfonic acid)‐l,2,4‐triazine] reacted with only small amounts of Fe3+, had a fairly wide range of Fe2+ detection (0–200 μg), P did not interfere with the reaction, and methodology was relatively simple. TPTZ [2,4,6‐tripyridyl‐s‐triazine] was very specific for Fe2+, did not react with Fe3+, had a relatively narrow range of Fe2+ detection (0–40 μg), P did not interfere with the reaction, and methodology was relatively simple. BPDS [4,7‐diphenyl 1.10‐phenanthrolinedisulfonic acid] reacted with a small amount of Fe3+, had a fairly wide range of Fe2+ detection (40–200 μg), did not react ...
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