Abstract
Checkpoint-independent scaling of the Saccharomyces cerevisiae DNA replication program
Highlights
In budding yeast, perturbations that prolong S phase lead to a proportionate delay in the activation times of most origins
These replication profiles were generated by microarray-based measurements of the DNA content of fluorescence-activated cell sorting (FACS)-sorted S phase cells (FACS profile in Figure S1, Additional file 1)
The profiles were strongly correlated with those of the single deletion profiles, showing the same scaling-like phenotype. Correlation of those profiles to the profile of the mrc1 deleted strain was low (Figures 3). These results indicate that the checkpoint is not required for the scaling of the replication profile with S phase duration and that the replication phenotype of mrc1 deleted cells is not connected to its role in mediating the checkpoint response
Summary
Perturbations that prolong S phase lead to a proportionate delay in the activation times of most origins. The DNA replication checkpoint was implicated in this scaling phenotype, as an intact checkpoint was shown to be required for the delayed activation of late origins in response to hydroxyurea treatment. Genome replication is initiated from multiple sites termed replication origins. The locations of those origins and their relative activation times during S phase are largely conserved between individual cells defining the DNA replication program [1,2,3,4]. While the replication program is clearly reproducible at the level of cell populations, it is not clear whether individual cells activate the same origins in the
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