Abstract
DNA purification is essential for the detection of human clinical specimens. A non-destructive, controllable, and low reagent consuming DNA extraction method is described. Negatively charged DNA is absorbed onto a negatively charged montmorillonite to achieve non-destructive DNA extraction based on cation bridge construction and electric double layer formation. Different valence cation modified montmorillonite forms were used to validate the charge-dependent nature of DNA adsorption on montmorillonite. Electric double layer thickness thinning/thickening with the high/lower valence cations exists, and the minerals tended to be sedimentation-stable due to the Van der Waals attraction/electrostatic repulsion. Li-modified montmorillonite with the lowest charge states showed the best DNA adsorption efficiency of 8–10 ng/μg. Charge-dependent regulating research provides a new perspective for controllable DNA extraction and a deep analysis of interface engineering mechanisms.
Highlights
DNA purification has been widely used for transfection, sequencing, and polymerase chain reaction (PCR)[1]
Negatively charged DNA is absorbed onto the negatively charged montmorillonite to achieve a non-destructive DNA extraction based on the cation bridge construction and the electric double layer formation (Fig. 1)
Different valence cations (Li+, Na+, Mg2+, Fe3+ and Al3+) with the same mass ratio were selected to modify the Ca-MMT to the maximum adsorption capacity, and the corresponding adsorption was measured
Summary
DNA purification has been widely used for transfection, sequencing, and polymerase chain reaction (PCR)[1]. The natural negative charge is derived from the crystalline substitution, and the charge state could be altered by electric double layer regulation or cation exchange. The positive charge facilitates adsorption by the strong electrostatic attraction between the opposite electricity. It is easy to see that the adsorption between two negative materials by weaker electrostatic attraction, such as a cation bridge, is more conducive to non-destructive desorption. Inspired by this idea, negatively charged DNA (purified from HeLa cells) is absorbed onto the negatively charged montmorillonite to achieve a non-destructive DNA extraction based on the cation bridge construction and the electric double layer formation (Fig. 1). The relationships among the charge, the dispersion, and the cation bridge for MMT interactions with DNA were analysed
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