Charge Detection Mass Spectrometry: What’s the “Big” Deal?

Abstract

Over the last several years, the landscape of the biopharmaceutical industry has begun to change. Monoclonal antibodies (mAbs) still dominate the pipelines in the biopharmaceutical industry; however, more complex molecules, such as antibody–drug conjugates (ADC), are becoming more predominant. In addition to the complexity of ADCs, one of the fastest growing classes of biopharmaceuticals is cell and gene therapies. Gene therapies, for example, use large viral vectors, such as adeno-associated viruses (AAV), as the preferred vector for performing gene therapy. With the complexity of biopharmaceuticals increasing, especially in their size, new and innovative approaches are needed to address and characterize these molecules. This month’s column reviews how charge detection mass spectrometry (CDMS) is being used to characterize some of these larger more complex molecules in the biopharmaceutical industry.