Abstract
The experimental model LEW.1WR1(1WR1) rats show increased type 1 diabetes (T1D) onset with the administration polyinosinic‐polycytidylic acid (PIC). The susceptibility window of inducible T1D in the LEW rat occurs between 21–35 days (3–5 weeks) of life, which is a similar life stage of the onset of human T1D (peak age of onset is 14 years old). The susceptibility of this model is related to FAT10 overexpression, a ubiquitin‐like protein that plays an unclear role in the regulation of lipid metabolism. The objective of this study was to compare the size and number of insulin stained pancreatic islets in 1WR1 rats treated with PIC with control rats during disease induction. Using immunohistochemistry we were able to visualize pancreatic islet infiltration by CD3+ and CD68+ stained cells. We observed increased immune cells in the PIC treated pancreata. There were also no significant differences in blood insulin level which appeared to be trending up. Using blinded trained scorers we observed islet size varied as well as intensity of staining. We believe that the beta cells may be responding to the inflammatory stimulus which could play a role in increasing the susceptibility to T1D.Support or Funding InformationThe University of Alabama in HuntsvilleThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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