Characterizing the chondrogenic niche in laser-engraved decellularized articular cartilage scaffolds

Abstract

Purpose: Among the different strategies to regenerate cartilage defects, the use of decellularized native cartilage has become of great interest since it bears the optimal characteristics to replace cartilage and support its regeneration. In a recent study, we have developed the first strategy to recellularize decellularized human articular cartilage by perforating the dense matrix by laser-engraving, facilitating cell ingrowth. Within the laser incisions of this material (CartiScaff) an increased differentiation was found, especially deep inside the tips of the incision, a phenomenon we designated as “tip-effect”.