Abstract
Soluble protein oligomers have only recently been shown to be a better predictor of cell cytotoxicity compared to insoluble fibrils, however the intrinsic heterogeneity of oligomers makes them difficult to characterize using ensemble biophysical techniques alone. For example, methods to separate proteins by size, such as SDS-PAGE or size exclusion chromatography, have largely been inadequate to describe oligomers, and do not recapitulate apparent oligomer sizes in electron micrographs. Similarly, the use of denaturing detergents (e.g., SDS) can actively induce protein oligomerization, thereby perturbing the states one wishes to measure.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
