Characterizing Pairwise U-Turn Behavior in Fish: A Data-Driven Analysis.

Abstract

We applied the time-series clustering method to analyze the trajectory data of rummy-nose tetra (Hemigrammus rhodostomus), with a particular focus on their spontaneous paired turning behavior. Firstly, an automated U-turn maneuver identification method was proposed to extract turning behaviors from the open trajectory data of two fish swimming in an annular tank. We revealed two distinct ways of pairwise U-turn swimming, named dominated turn and non-dominated turn. Upon comparison, the dominated turn is smoother and more efficient, with a fixed leader-follower relationship, i.e., the leader dominates the turning process. Because these two distinct ways corresponded to different patterns of turning feature parameters over time, we incorporated the Toeplitz inverse covariance-based clustering (TICC) method to gain deeper insights into this process. Pairwise turning behavior was decomposed into some elemental state compositions. Specifically, we found that the main influencing factor for a spontaneous U-turn is collision avoidance with the wall. In dominated turn, when inter-individual distances were appropriate, fish adjusted their positions and movement directions to achieve turning. Conversely, in closely spaced non-dominated turn, various factors such as changes in distance, velocity, and movement direction resulted in more complex behaviors. The purpose of our study is to integrate common location-based analysis methods with time-series clustering methods to analyze biological behavioral data. The study provides valuable insights into the U-turn behavior, motion characteristics, and decision factors of rummy-nose tetra during pairwise swimming. Additionally, the study extends the analysis of fish interaction features through the application of time-series clustering methods, offering a fresh perspective for the analysis of biological collective data.