Characterizing Circular RNAs Using Nanopore Sequencing

Abstract

The reconstruction of full-length sequences of circular RNAs (circRNAs) provides important information for circRNA prioritization and function prediction. circRNA identifier using long-read sequencing data (CIRI-long) is a comprehensive experimental and computational approach to determine full-length circRNA isoforms using nanopore sequencing. Using rolling circular reverse transcription, CIRI-long constructs long cDNA libraries containing multiple full-length template sequences and characterizes circRNA structures using a k-mer based strategy.