Characterizations, physio-chemical and biological evaluation of novel nanostructure hydroxyapatite synthesized by conventional method using egg shell and by green synthesis method using Melia dubia extract

Abstract

• The green synthesis of Hydroxyapatite nanorods using Melia dubia leaf extract can be employed in better way than conventional synthesized one in biological, electronic and mechano use. • This study has proved the possibility of producing inexpensive, non-toxic and ecofriendly HA and other calcium phosphate nanoparticles solely from Melia dubia . • The green synthesized HA nanorods had greater antibacterial activity against Gram positive and Gram negative bacteria. • The electronic influx and frequency, voltage augmentation of HA and GHA gives the superiority by GHA and tribological data gives 325 MPa for GHA as the better one than normal and HA. Nanomaterials based on hydroxyapatite (HA) are well synthesized in the traditional mode using reagents, are not ecological friendly and are of very expensive one. Since current research areas are well focused on the production/synthesis of nanomaterials using an environmental friendly approach, the synthesis of green HA using plant extracts is of paramount importance. In this context, this work is carried out for the chemical and green synthesis of HA and GHA nanorods, from egg shells and Melia dubia as well as for the characterization of their structural, morphological, thermal, optical XRD, EDAX, SEM, and FT-IR analysis as well as in mechano and in influx for filter uses. Antimicrobial properties of these nanorods are evaluated. GHA nanorods are made using the herb Melia dubia . HA and GHA are evaluated for antimicrobial properties against Staphylococcus aureus, Enterococcus faecalis, Proteus vulgaris and Escherichia coli . The results showed that GHA exhibits a strong antibacterial effect against all bacteria studied. GHA nanoparticles can be used for various applications due to their environmental friendliness, non-toxicity and compatibility in bio and especially in tribological and electronics.