Characterizations of general esterases in relation to malathion susceptibility in two field populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen)

Abstract

General esterases were characterized and compared from two populations of the oriental migratory locust, Locusta migratoria manilensis, collected from Huanghua and Pingshan Counties, Hebei Province, China. General esterases were most concentrated in the thorax and abdomen, which contained 46.1 and 36.1% of total esterase activity in females, and 42.7 and 36.0% in males, respectively, when α-naphthyl acetate was used as a substrate. There was no distinct difference in esterase banding patterns in different body regions for the substrates α-naphthyl acetate and β-naphthyl acetate on non-denaturing polyacrylamide gel electrophoresis (PAGE). However, the general esterase activities in the Huanghua population were 1.8-fold higher than those in the Pingshan population in both females and males. Increased esterase activity in the Huanghua population appeared to be mainly due to several additional esterase bands detected on non-denaturing PAGE. Inhibition studies of general esterases using four inhibitors, including paraoxon, malaoxon, eserine, and carbaryl, indicated that most general esterases in the two populations were B-type. The increased esterase activity in the Huanghua population appeared to be associated with a 1.8-fold decreased susceptibility to malathion. Such differences may attribute to the difference in control practices for the locust between Huanghua and Pingshan Counties.