Abstract

Ten strains of Fusarium solani were isolated on Potato Dextrose Agar medium from rotten potato tubers marketed in Upper Egypt region. The identification of collected isolates was carried out by morphological criteria and species-specific primer, TEF-Fs4f and TEF-Fs4r was used to confirm the identification. Genetic diversity of the collected isolates studied by using specific genes random primer polymerase chain reaction (SGRP-PCR) techniques. The results indicated that F. solani isolates have high genetic diversity and no correlation between the geographical site and banding patterns was recorded. The pathogenicity test showed that 20% of F. solani isolates registered as highly pathogenic. No resemblance was detected between pathogenicity levels and banding patterns of SGRP obtained from F. solani isolates. All F. solani isolates exhibited amylolytic, cellulolytic, pectinolytic, lipolytic and proteolytic activities and the amount of each enzyme significantly different among F. solani isolates.

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