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Abstract
ThePerina nudanucleopolyhedrovirus (PenuNPV) polyhedrin gene was located inEcoRI-G (6.3 kilobase pairs; kbp) andPstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV polyhedrin was most closely related toOrgyia pseudotsugatamultiple NPV(OpMNPV) polyhedrin.
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