Abstract
We have pharmacologically characterized voltage sensitive calcium channels (VSCCs) in GH 3 cells, an anterior pituitary clonal cell line known to secrete prolactin and growth hormone. Raising the medium K + concentration from 5 to 50 mM caused an immediate increase in net 45Ca 2+ uptake which remained apparent over a 15 minute time course. 45Ca 2+ uptake was maximally stimulated nearly 10-fold over basal levels. This K +-induced stimulation of Ca 2+ uptake was not prevented by 10 −5M tetrodotoxin or by replacing sodium with choline in the assay medium. Ca 2+ uptake was, however, inhibited by several VSCC antagonists: nitrendipine, D-600, diltiazem and Cd 2+. Further, the novel dihydropyridine VSCC agonists, BAY K8644 and CGP 28392, enhanced 50 mM K +-stimulated 45Ca 2+ uptake and these effects were blocked by nitrendipine.
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