Abstract
The protein, phospholipid and carbohydrate components of vitellin and vitellogenin from Rhodnius prolixus were analyzed after being labelled by feeding the insects with blood enriched with 32P i. The carbohydrate moiety is comprised mainly of mannose residue (90%), but also contains glucose and traces of galactose and glucosamine. Both vitellin and vitellogenin are present in two different aggregational states that differ in molecular weight. Each of the individual aggregates releases, under denaturing conditions, the same four polypeptides with approximate molecular weights of 180, 158, 44 and 38 K daltons with no consistent stoichiometries. Analysis of the phosphorylated yolk protein after specific precipitation with serum against vitellin showed 62% of the total radioactivity in the phospholipid fraction and 38% in the glycoprotein fraction. Upon further analysis by thin layer chromatography, the following phospholipids were found: phosphatidylcholine (64.2%), phosphatidylethanolamine (30%), phosphatidic acid (4,4%), sphingomyelin (0.7%), phosphatidylserine (0.6%) and cardiolipin (0.2%). Analysis of the glycoprotein fraction showed that all four subunits were phosphorylated, part of the radioactivity being found as [ 32P]serine and [ 32P]mannose.
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