Abstract
We present here the isolation and characterization of Acinetobacter pittii phage vB_ApiM_fHyAci03 (fHyAci03), which belongs to the family Myoviridae. The fHyAci03 genome was found to be 165,975 bp in length and predicted to contain 255 genes. While the whole genome was 92.4% identical to Acinetobacter baumannii phage KARL-1, phylogenetic analysis based on phage long distal tail fiber amino acid sequences assigned fHyAci03 and KARL-1 to different subclusters, reflecting their different host species. Together with phylogenetic analysis, genome comparisons indicated that fHyAci03 is a novel member of the subfamily Tevenvirinae. Host range experiments revealed that fHyAci03 could infect two clinical strains of Acinetobacter nosocomialis and six clinical strains of A. pittii. Thus, fHyAci03 is a novel lytic phage that infects clinical Acinetobacter strains and represents a potential new candidate to be used in phage therapy.
Highlights
The genus Acinetobacter includes multiple nosocomial opportunistic pathogens
FHyAci03 was isolated from a municipal sewage sample collected in Hyvinkää, Finland, using clinical A. pittii strain #5565 as the host
Together with the genomic information, the morphological characteristics indicated that fHyAci03 belongs to the family Myoviridae and the subfamily Tevenvirinae. fHyAci03 host range was tested with 48 clinical Acinetobacter strains (Supplementary Table 1)
Summary
The genus Acinetobacter includes multiple nosocomial opportunistic pathogens. Within this genus, A. baumannii, A. pittii, and A. nosocomialis are the most frequently isolated species from hospitalized patients around the world [1]. A. baumannii, A. pittii, and A. nosocomialis are the most frequently isolated species from hospitalized patients around the world [1]. FHyAci03 was isolated from a municipal sewage sample collected in Hyvinkää, Finland, using clinical A. pittii strain #5565 (obtained from HUSLAB, Helsinki, Finland) as the host.
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