Characterization of valine transport in sea urchin eggs

Abstract

In unfertilized eggs, the mechanism of valine uptake can be summarized as follows. It is saturable over the external concentration of valine and insensitive to the presence of external sodium, depletion of cellular energy supplies and intracellular acidosis. The activation energy for the transport reaction (16.3 kcal/mol) is within the range of values reported for active transport of small molecules. In fertilized eggs, the total rate of valine uptake can be divided into two components: (i) a Na +-insensitive uptake which accounts for about 7% of total absorption as shown by studies in Na +-free medium seems to possess the same characteristics as in unfertilized eggs, (ii) a Na +-dependent transport of valine which constitutes the main entry is formed about 5 min after fertilization. It follows Michaelis-Menten kinetics characterized by 15-fold increase in V max with no change in K m . These two mechanisms have characteristics in common, such as their insensitivity to metabolic energy supply, their energy of activation and their ability to concentrate valine. The relationship between the establishment of the Na +-dependent valine uptake and the ionic events triggered by fertilization is discussed.