Characterization of urban aerosol: Seasonal variation of genotoxicity of the water-soluble portion of PM2.5 and PM1

Abstract

Urban particulate matter (PM) is a complex mixture of several classes of chemicals: elemental carbon, ammonium, sulfates, nitrates, organic compounds and metals. For a long time, numerous studies had shown that PM causes health problems and, in 2013, it has been classified by the International Agency for Research on Cancer as carcinogenic to humans (group 1). Furthermore, it’s known that the fine fraction of PM is the most genotoxic, and that smaller particles are retained by the lower respiratory system, making fine particles a public health concern. In this study we characterize the water-soluble portion of urban aerosol from Bologna, a county town of Emilia-Romagna in the north of Italy, by collecting the finest fractions of airborne particles, PM2.5 and PM1, in three different seasons (winter, summer and autumn) over a three-year period. The genotoxicity of the water-soluble extracts was evaluated, both by a standard and a enzyme-modified Comet assay and also by the Micronucleus test, with lung adenocarcinoma epithelial cells (A549). In the same extracts, water-soluble metals (V, Ni, Cu, Cr, Fe) were detected and associations between the physicochemical parameters of PM and genotoxicity were evaluated. DNA strand breaks were found in summer and winter samples in the Comet experiments, whereas oxidative damage was induced by autumn extracts; winter samples induced chromosome breakage or loss in A549 cells. Iron and copper were the most abundant transition metals in both fractions and both were associated with micronuclei induction, whereas chromium is linked with oxidative damage. This study also shows that the water-soluble fraction of PM contributes to global genotoxicity and that transition metals play a role, therefore both organic and water-soluble fractions should be considered in an air-quality monitoring program.