Abstract
Acoustic field parameters were measured and calculated for an automatic-scan ultrasonic exposure apparatus designed for biological effects studies that has been previously reported. Free far-field pressure profiles of disk transducers resonant at 1.0 MHz were obtained under conditions using a hydrophone calibrated by a pendulum consisting of a stainless steel sphere on a bifilar suspension. Typical total acoustic power measurements using a radiation force balance technique agreed within ±10% of the integrated intensity profiles. An efficient computer program was used to calculate the pressure distribution for a baffled piston by means of an existing Fourier transform approach. These theoretical intensity profiles agreed well with the actual data. Several scans directly behind holders used to expose both biological solutions and cell suspensions showed disruption of the normal free-field pattern. Scans also demonstrated differences in the acoustic coupling to mice for various preparation methods prior to ultrasound exposure.
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