Abstract
1. 1. Chromatin of sea urchin embryos was chromatographed on a hydroxyapatite column, and the two largest fractions of proteins desorbed with 0.05 M and 0.2 M Na-phosphate, pH 6.8, were comparatively characterized. 2. 2. The mol. wt ranges of these fractions were about 18,000 and 14,000 daltons, respectively, at both molarities of the phosphate buffer. 3. 3. Both fractions at each elution molarity were found to be acidic, and to differ somewhat in their respective amino acid compositions. No important differences related to the stage of development could be detected in this respect. 4. 4. Upon re-electrophoresis both large fractions resolved in one major and three minor bands. The amino acid composition of the major band was different for the blastula and gastrula chromatin. 5. 5. It is likely that these fractions contain glycoprotein subunits.
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