Abstract
Myo‐inositol phosphates (InsPs) are critical signaling molecules used by eukaryotes, with roles in nutrient and abiotic stress sensing/signaling. The newest members of this family are the inositol pyrophosphates which contain diphospho‐ or triphospho‐ (PPx) moieties at one or more positions on the inositol ring. These molecules have recently been identified across the plant kingdom and their synthesis in Arabidopsis is the result of two highly similar genes, AtVIP1 and AtVIP2. We report the spatial/temporal expression of these genes using real‐time PCR and promoter:GUS analysis and the subcellular localization of the VIP proteins, using transient expression of GFP fusion proteins in Nicotiana benthamiana. Both VIPs are localized in the endoplasmic reticulum, with AtVIP1 additionally localizing to the nucleus. These studies further our understanding of the distinct role each VIP may play in the plant. Mutants lacking expression of both AtVIP1 and AtVIP2 genes have been identified and are viable. Work is on‐going to determine the impact of inositol pyrophosphate synthesis in these double mutants.
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