Abstract
When grown on methanol or methylamine, Paracoccus denitrificans synthesized three periplasmic soluble c-type cytochromes, cytochrome c550 and two additional cytochromes which were not present during growth on succinate and have not previously been characterized. These cytochromes have been separated from each other and their physical properties have been determined. The inducible cytochromes, c551i and c553i, exhibit Mr and pI values of 22,000 and 3.5, and 30,000 and 3.8, respectively. Cytochrome c553i binds CO. None of these cytochromes accepted electrons directly from methylamine dehydrogenase, but each accepted electrons from amicyanin, the electron acceptor for methylamine dehydrogenase. Cytochrome c551i was the most efficient electron acceptor for amicyanin, exhibiting a half-time of reaction at least 6 and 15 times faster than those observed for cytochromes c553i and c550, respectively.
Highlights
The sample was applied to a DEAE-cellulose column which had been equilibrated with the dialysis buffer, and was eluted with a linear gradient of 0‘400 nM NaCI in the starting buffer
Cytochrome cSS8eluted between the two dehydrogenases and cytochrome cSSI1eluted immediately after methylamine dehydrogenase. Pooled fractions of the latter two cytochromes were further purified by passage over Sephadex G-75 to remove any methanol and methylamine dehydrogenases Paracoccus denitrificansis capable of growth with methanol whichwere present
P.denitrificans is a periplasmic Type I blue copper protein, lysozyme (14,000).Heme stains were performed according to the amicyanin [3, 5], which, like methylamine dehydrogenase, is induced during growth on methylaminebut not present during method of Francis and Becker [11]
Summary
Administration Medical Center, San Francisco, California zation of these periplasmic C-type cytochromes of P. denitri- When grown on methanol or methylamine, Paracoccus denitrificanssynthesized three periplasmic soluble c-type cytochromes, cytochrome C660 and two additional cytochromes which were not present during growth on succinate and have not previously been characterized. Cytochrome C661i was the most efficient electron acceptor for amicyanin, exhibiting a half-time of reaction at least 6 and 15 times faster than those observed for cytochromes c663i and
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