Abstract
Chromogenic media are frequently used in direct and rapid identification of yeasts because different organisms could produce unique colors and specific colony morphology on these media. A total of 273 clinical isolates and 8 reference strains of yeasts were used to evaluate the performance of two chromogenic media, Candida ID2 and CHROMagar Candida, on the identification of various yeasts. The fertility of both media was 100%, but selectivity was 86.5% and 96.2%, respectively. Sensitivities and specificities of the 5 most clinically important Candida species on Candida ID2 were as follows: 100% and 95.3% for Candida albicans, 100% and 93.8% for C. glabrata, 90.0% and 82.7% for C. parapsilosis, 95.2% and 79.6% for C. tropicalis, and 97.2% and 84.5% for C. intermedia. For CHROMagar Candida, the figures were 100% and 94.6% for C. albicans, 90.2% and 95.4% for C. glabrata, 90% and 82.3% for C. parapsilosis, 100% and 78.8% for C. tropicalis, and 100% and 83.7% for C. intermedia. In conclusion, Candida ID 2 appears to have a better sensitivity than CHROMagar Candida in the detection and identification of yeasts.
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