Abstract
AbstractMadeira laurel oil was fractionated by liquid extraction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization‐MS (APCI‐MS). Eluted molecular species compositions of the eluted TAG in the complex natural mixture were determined by GC identification of FAME and byLC‐atmospheric pressure chemical ionization (APCI)‐MS analysis of the lipid. The APCI‐MS spectra of most TAG exhibited [M+H]+ and [M−RCOO]+ ions, which defined the M.W. and the molecular association of fatty acyl residues, respectively. Despite the relatively high degree of saturation, with a saturated/unsaturated ratio of 0.70, no totally saturated TAG nor mixed asymmetric TAG with two saturated FA (SSM or SSU, where S is saturated, M is monounsaturated, and U is unsaturated) were found. This type of molecular structure provides a possible explanation for the relatively low m.p. (12–15°C) and also the high oxidative resistance observed.
Highlights
Madeira laurel oil was fractionated by liquid extraction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization-MS (APCI-MS)
Madeira laurel oil is a “natural fruit juice” in the sense that both are obtained from the fruit of a tree solely by mechanical or other physical processes; the berries of L. novocanariensis are similar in aspect to wild olives and from them a fatty oil is extracted according to procedures quite similar to primitive olive oil production
The major compounds are TAG, present in 95–98% (3,4); in Madeira laurel oil, TAG account for 85%, the other 15% being composed of hydrocarbons, lactones, sterols, and wax esters
Summary
Madeira laurel oil was fractionated by liquid extraction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization-MS (APCI-MS). The sn-1 and sn-3 positions of TAG cannot be distinguished, by means of MS, (11), the FA combinations and regioisomeric structure of most ACN/n (acyl carbon number/number of double bonds) species can be rapidly determined with minimal sample preparation, which is not achieved by other methods.
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