Abstract
Abstract Objective: Three plasmids from Lactobacillus paracasei 54 were isolated from healthy newborn infant fecal samples. Methods: Plasmid was extracted using an AxyPrep Plasmid Miniprep Kit and lysozyme. The extracted plasmids were sequenced using a Roche 454 Genome Sequencer FLX. Results: Three plasmids were isolated from Lactobacillus paracasei 54. These plasmids are designated pLP5401- 03, and they are 9754, 6650, and 1788 bp in size. Conclusion: Plasmids pLP5401 and pLP5402 were found to contain replication genes that are likely to function via the theta-type mechanism. Plasmid pLP5403 is predicted to replicate via the rolling-circle replication (RCR) mechanism. The RCR replication plasmid can be applied as a useful vector in the food industry
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