Characterization of three cell lines established from medaka embryos

Abstract

We established three cell lines from medaka embryos. In the present study, we investigated the optimal conditions for growth of these cells with regard to the basal culture medium and concentration of fetal bovine serum (FBS). The best cell growth for all three cell lines was achieved with a 1:1 mixture of L15 medium and 199 medium with a relatively high concentration (20%) of FBS. The growth curves for the cell lines revealed that their doubling times ranged from 48 to 93 h. We also analyzed the numbers of chromosomes in the cell lines and found that their numbers varied from near-diploid to hyper-tetraploid. The results of the present study should be helpful for research in fish cell culture.