Abstract
Allis shad Alosa alosa and twaite shad Alosa fallax are two related anadromous European clupeid species which have undergone a sharp decline in the past decades. We describe the development of 13 microsatellite markers for both species, based on 454 pyrosequencing technology. The number of alleles per locus ranged from 2 to 11 in A. alosa and from 4 to 13 in A. fallax. Gene diversity ranged from 0.364 to 0.800 for A. alosa and 0.226 to 0.849 for A. fallax. These markers will help in conservation genetics studies such as assessing the extent of population decline, defining appropriate conservation units and monitoring reintroduction programs.
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