Abstract
The products of the Nbs1, Mre11, and Rad50 genes form the MRN complex. This complex is involved mainly in DNA repair, but it also has roles in meiosis, replication, telomere maintenance, and cell cycle checkpoint signaling. Its study is therefore of great importance if our knowledge of these important cell processes is to improve. To date, few studies have examined the MRN complex in plants. The present work describes the molecular characteristics of the Nbs1 gene in diploid and polyploid species of wheat. The expression of MRN complex homologous genes in meiocytes and in somatic cells treated with genotoxic agents is also examined. In addition, the expression of MRN complex homoeologous genes is analyzed in polyploid wheat species to determine the function of the duplicated genes. The characterization of Nbs1 in wheat species revealed its low sequence homology with the Nbs1 gene of other organisms. However, the homologous Nbs1 genes of wheat showed very great similarity. All the Nbs1 wheat genes examined...
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