Characterization of the whole chloroplast genome of a rare and endangered species Aconitum reclinatum (Ranunculaceae) in the United States

Abstract

The complete chloroplast genome (cp genome) of Aconitum reclinatum (Ranunculaceae) was characterized through Illumina paired-end sequencing. The cp genome is circle and 157,354 bp in length, consisting of a pair of 26,061 bp inverted repeat regions (IRs) which are separated by a large single copy region (LSC) of 88,269 bp and a small single copy region (SSC) of 16,963 bp. The cp genome contains 135 genes, including 87 protein-coding genes (PCGs), 40 tRNA genes (tRNA) and eight ribosomal RNA genes (rRNA). Among these, seven PCGs, eight tRNA and four rRNA are duplicated. The overall GC content of the A. reclinatum cp genome is 38.00%, while the corresponding values of the LSC, SSC and IRs regions are 36.00, 32.80 and 43.00%, respectively. The phylogenetic analysis suggested that A. reclinatum is closely related to A. barbatum var. hispidum and A. barbatum var. puberulum with high bootstrap support of 100%.