Abstract
Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng (P. huashania)-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. Sequential genomic in situ hybridization (GISH) and multicolor fluorescence in situ hybridization (mc-FISH) analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D. Expressed sequence tag-sequence tagged site (EST-STS) marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line. In addition, the H139 line was shown to be cytologically stable with a dwarf phenotype and increased spikelet number. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding.
Highlights
Wheat (Triticum aestivum L.) is one of the most important global food crops, contributing nearly a fifth of the total calories consumed by humans (International Wheat Genome Sequencing Consortium (IWGSC), 2018)
The wheat take-all disease susceptibility test on seedlings was completed in an artificial climate cabinet with controlled temperature, humidity, and light conditions
The diseaseresistant control oat (Bayou 8) displayed complete immunity to wheat take-all disease, whereas the susceptible control Yangmai 158 was sufficiently infected with an average disease index (ADI) of 59.4%
Summary
Wheat (Triticum aestivum L.) is one of the most important global food crops, contributing nearly a fifth of the total calories consumed by humans (International Wheat Genome Sequencing Consortium (IWGSC), 2018). P. huashania is primarily distributed in the Huashan section of the Qinling Mountains, China (Kuo, 1987; Baden, 1991) This species is favored by researchers because of its cold and drought tolerance, early maturity, perennial traits, dwarf stature, and resistance to wheat take-all (G. tritici), stripe rust Wang and Shang (2000) used a series of wheat-P. huashania-derived offspring to screen for resistance to take-all disease at the seedling stage. This revealed that P. huashania displayed high disease resistance, whereas the wheat parental line 7182 was susceptible. We characterized a wheat-P. huashanica derived line H139 that displays wheat take-all disease resistance at both the seedlings and mature stages. Our results indicate that the H139 germplasm resource can serve as bridge material for wheat improvement
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