Abstract
C-type lectins (CTLs), a superfamily of calcium-dependent carbohydrate binding proteins, which remain uncharacterized in bivalve mollusks such as blood clam Tegillarca granosa, plays a pivotal role in non-self-recognition and pathogen clearance within the innate immune system. In this study, we identified a CTL as a potential marker gene for immune cells in T. granosa using single-cell RNA-sequence (scRNA-seq). Structural predictions confirmed TgCTL as a functional protein, being characterized by a classic C-type lectin domain (CTLD) and a stable transmembrane structure. Furthermore, the Vibrio parahaemolyticus challenge experiment indicated that the TgCTL gene can significantly respond to pathogen invasion as a marker of immune response and can effectively eliminate pathogens. Results of RNA interference (RNAi) showed that TgCTL knockdown led to a significant decrease in hemocytes phagocytic activity (P < 0.05). Taken together, the results of this study revealed that TgCTL, a marker for immune response, could participate in immune defense against pathogens by enhancing phagocytosis of hemocytes, providing new insights into the antimicrobial immune response of T. granosa.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
