Abstract
Etiolation (a process of growing plants in partial or complete absence of light) promotes adventitious root formation in tetraploid black locust (Robinia pseudoacacia L.) cuttings. We investigated the mechanism underlying how etiolation treatment promotes adventitious root formation in tetraploid black locust and assessed global transcriptional changes after etiolation treatment. Solexa paired-end sequencing of complementary DNAs (cDNAs) from control (non-etiolated, NE) and etiolated (E) samples resulted in 107,564 unigenes. In total, 52,590 transcripts were annotated and 474 transcripts (211 upregulated and 263 downregulated) potentially involved in etiolation were differentially regulated. These genes were associated with hormone metabolism and response, photosynthesis, signaling pathways, and starch and sucrose metabolism. In addition, we also found significant differences of phytohormone contents, activity of following enzymes i.e., peroxidase, polyphenol oxidase and indole acetic acid oxidase between NE and E tissues during some cottage periods. The genes responsive to etiolation stimulus identified in this study will provide the base for further understanding how etiolation triggers adventitious roots formation in tetraploid black locus.
Highlights
Tetraploid black locust (Robinia pseudoacacia L.) is produced by artificially doubling the chromosomes of a diploid black locust, and the species was first imported from South Korea intoChina in 1997 [1]
The goal of this study was to provide a theoretical basis for the molecular mechanisms underlying etiolated promotion of adventitious root development of softwood cuttings in tetraploid black locust
Several studies have focused on the mechanisms by which etiolation improves adventitious root formation of stem cuttings
Summary
Tetraploid black locust (Robinia pseudoacacia L.) is produced by artificially doubling the chromosomes of a diploid black locust, and the species was first imported from South Korea intoChina in 1997 [1]. Tetraploid black locust (Robinia pseudoacacia L.) is produced by artificially doubling the chromosomes of a diploid black locust, and the species was first imported from South Korea into. Compared to the normal diploid Robinia pseudoacacia, tetraploid R. pseudoacacia has a significantly higher yield with larger leaves and higher leaf protein content. It is polyanthus, long blossoming, and suitable for feeding and beekeeping. Tetraploid R. pseudoacacia is fast growing and able to tolerate harsh environments including salt, drought, and nutrient deficiencies. It has high ecological value and is widely planted to improve the soil [2].
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