Abstract
Endometrium receptivity is essential for successful embryo implantation in mammals. However, the lack of genetic information remains an obstacle to understanding the mechanisms underlying the development of a receptive endometrium from the pre-receptive phase in dairy goats. In this study, more than 4 billion high-quality reads were generated and de novo assembled into 102,441 unigenes; these unigenes were annotated using published databases. A total of 3,255 unigenes that were differentially expressed (DEGs) between the PE and RE were discovered in this study (P-values < 0.05). In addition, 76,729–77,102 putative SNPs and 12,837 SSRs were discovered in this study. Bioinformatics analysis of the DEGs revealed a number of biological processes and pathways that are potentially involved in the establishment of the RE, notably including the GO terms proteolysis, apoptosis, and cell adhesion and the KEGG pathways Cell cycle and extracellular matrix (ECM)-receptor interaction. We speculated that ADCY8, VCAN, SPOCK1, THBS1, and THBS2 may play important roles in the development of endometrial receptivity. The de novo assembly provided a good starting point and will serve as a valuable resource for further investigations into endometrium receptivity in dairy goats and future studies on the genomes of goats and other related mammals.
Highlights
Embryo implantation is a complex initial step in the establishment of successful pregnancy in mammals[1] and consists of apposition, adhesion and invasion[2]
This study used RNA sequencing (RNA-Seq) to compare the transcriptomic landscapes of the endometrium from the pre-receptive endometrium (PE) and receptive endometrium (RE) phases of 20 healthy, 24-month-old multiparous dairy goats
Investigating the transcriptome profile of the receptive and pre-receptive endometrium will contribute to our understanding of the biochemical and physiological development of endometrial receptivity during the “window of implantation”
Summary
Embryo implantation is a complex initial step in the establishment of successful pregnancy in mammals[1] and consists of apposition, adhesion and invasion[2]. We constructed a comprehensive analysis of the endometrial transcriptional profiles at the global level to compare the genes expressed in the PE and RE and further explore DEGs, single nucleotide polymorphisms (SNP) and simple sequence repeat (SSR) using Gene Ontology (GO) and Kyoto Encyclopedia of Genes (KEGG) for DEGs. the results of our present study may provide essential information in support of further research on the development of endometrial receptivity in dairy goats.
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