Abstract
The HDV ribozyme’s folding pathway is, by far, the most complex folding pathway elucidated to date for a small ribozyme. It includes 6 different steps that have been shown to occur before the chemical cleavage. It is likely that other steps remain to be discovered. One of the most critical of these unknown steps is the formation of the trans Watson-Crick GU base pair within loop III. The U23 and G28 nucleotides that form this base pair are perfectly conserved in all natural variants of the HDV ribozyme, and therefore are considered as being part of the signature of HDV-like ribozymes. Both the formation and the transformation of this base pair have been studied mainly by crystal structure and by molecular dynamic simulations. In order to obtain physical support for the formation of this base pair in solution, a set of experiments, including direct mutagenesis, the site-specific substitution of chemical groups, kinetic studies, chemical probing and magnesium-induced cleavage, were performed with the specific goal of characterizing this trans Watson-Crick GU base pair in an antigenomic HDV ribozyme. Both U23 and G28 can be substituted for nucleotides that likely preserve some of the H-bond interactions present before and after the cleavage step. The formation of the more stable trans Watson-Crick base pair is shown to be a post-cleavage event, while a possibly weaker trans Watson-Crick/Hoogsteen interaction seems to form before the cleavage step. The formation of this unusually stable post-cleavage base pair may act as a driving force on the chemical cleavage by favouring the formation of a more stable ground state of the product-ribozyme complex. To our knowledge, this represents the first demonstration of a potential stabilising role of a post-cleavage conformational switch event in a ribozyme-catalyzed reaction.
Highlights
Understanding the RNA structure/function relationship is critically important for further development in the fields of both molecular and cellular biology
The reactions were incubated for 2 h at 37uC in the presence of 10 mM MgCl2, and the reaction products analysed by denaturing polyacrylamide gel electrophoreses (PAGE)
It is noteworthy that a trans Watson-Crick/Hoogsteen (tWH) U23/G28 base pair, in which the G was found to be in an anti conformation, was retrieved in the pre-cleaved genomic hepatitis delta virus (HDV) Rz crystal [7]
Summary
Understanding the RNA structure/function relationship is critically important for further development in the fields of both molecular and cellular biology. Conversion of the HDV self-cleaving strand from a cis-acting to a trans-acting system by separating the Rz and substrate (S) domains significantly facilitated its study (Figure 1A) Both structure/function assays and structural studies have helped to elucidate its double-pseudoknot secondary structure, a structure that is composed of two stems (stems I and II, the latter forming a pseudoknot in the cis-acting version), two stem-loops (III and IV) and three single-stranded junctions (I/II, I/IV, and IV/II) (Figure 1A) [2]. Both junction I/IV and loop III are single-stranded in the initial stages of folding, but subsequently become involved in the formation of the pseudoknot I.I [3]
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