Abstract
Introduction: In recent decades, the antimicrobial surfaces/coating properties towards a longlasting microbicidal effect have drawn enormous attention by researchers, they have been developed and used in a wide variety of high-touch hospital devices as a potential approach.
 Methods: In this work, Ag NPs was synthesized by sputtering method at the different annealing temperatures of 100◦C, 200◦C, 300◦C, and 400◦C.
 Results: As a result, the as-synthesized Ag-300 exhibits the highest E. coli antibacterial performance compared with others. This can be attributed to the change of the Ag NPs toxicity based on the growth of nanoparticles during the deposition process related to the Ostwald ripening process, thermal activation and coalescence particles.
 Conclusion: This work provides an essential insight into the antimicrobial activity of Ag NPs-based films synthesized through the vacuum deposition technique, resulting in opening a new approach for enhancing the antimicrobial efficacy and prospects.
 
Highlights
In recent decades, the antimicrobial surfaces/coating properties towards a longlasting microbicidal effect have drawn enormous attention by researchers, they have been developed and used in a wide variety of high-touch hospital devices as a potential approach
Previous studies reported that Ag NPs, which synthesized in a similar way to form silver ions, exerted against bacteria through a multifactorial process, and they were associated with inhibiting the growth of harmful bacteria as they were harm to the bacterial cell wall and plasma membrane or restraint on DNA replication and protein 8
We have investigated the antimicrobial activity of Ag NPs films prepared by DC magnetron sputtering technique
Summary
The antimicrobial surfaces/coating properties towards a longlasting microbicidal effect have drawn enormous attention by researchers, they have been developed and used in a wide variety of high-touch hospital devices as a potential approach. Previous studies reported that Ag NPs, which synthesized in a similar way to form silver ions, exerted against bacteria through a multifactorial process, and they were associated with inhibiting the growth of harmful bacteria as they were harm to the bacterial cell wall and plasma membrane or restraint on DNA replication and protein 8 They could be released by the natural formation of ion Ag in the presence of reductive components in the environment 9. The characteristic features of nanoparticles such as size, shape, density distribution have been demonstrated to affect the antibacterial activity of Ag NPs significantly, this could be attributed to a differential release of Ag+ ions 10 Previous researches in this field suggested that the effects of shapes and sizes prepared by wet chemical reduction methods or biosynthesis play a significant role in the antimicrobial nature of Ag NPs 11–14.
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