Abstract
Micromolar concentrations of ATP induced a cAMP-independent glycogenolytic response in rabbit and guinea pig hepatocytes. With ATPα[ 35S] (adenosine 5′-[α[ 35S]thio]triphosphate) as radioligand, we detected the presence of specific purinoceptors on hepatocytes and liver plasma membranes of both species. We determined a K d value of 0.28 μM and a B max of 4.8 pmol/10 6 cells for rabbit hepatocytes and a K d of 0.25 μM and a B max of 7.0 pmol/10 6 cells for guinea pig hepatocytes. The K d values with purified plasma membranes from rabbit and guinea pig liver, were respectively 0.2 and 0.1 μM whereas the B max values were respectively 71 and 47 pmol/mg of protein. These purinoceptors belong to the P 2Y-subclass as is evidenced by the high degree of similarity which exists between the binding affinities of several ATP analogues to either rabbit or guinea pig liver plasma membranes and rat liver plasma membranes, previously shown to possess P 2Y-purinoceptors.
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