Abstract
Extracellular polymeric substances (EPS) play major roles in the efficacy of biofilms such as anaerobic granules, ranging from structural stability to more specific functions. The EPS of three granular anaerobic sludges of different origins were studied and compared. Particularly, the peptides from the protein fraction were identified by mass spectrometry. Desulfoglaeba and Treponema bacterial genera and Methanosaeta and Methanobacterium archaeal genera were prominent in all three sludges. Methanosaeta concilii proteins were the most represented in EPS of all three sludges studied. Principally, four proteins found in the three sludges, the S-layer protein, the CO-methylating acetyl-CoA synthase, an ABC transporter substrate-binding protein and the methyl-coenzyme M reductase, were expressed by Methanosaeta concilii. Mainly catabolic enzymes were found from the 45 proteins identified in the protein fraction of EPS. This suggests that EPS may have a role in allowing extracellular catabolic reactions.
Highlights
The biofilm is the best-known community lifestyle for microorganisms
This study focuses on comparison of three anaerobic granular sludges treating different industrial effluents
Microbial populations The molecular-based profiles of the microbial communities were first determined in the three anaerobic sludges under investigation (i.e. Agropur, cheese factory; Lassonde, juice industry; Tembec, paper mill), using high throughput screening (HTS)
Summary
The biofilm is the best-known community lifestyle for microorganisms. Extracellular polymeric substances (EPS) embed biofilm cells, play an important role in the biofilm development and cohesion. The EPS macromolecules are either excreted by microorganisms, produced from cell lysis, or adsorbed from the external environment (e.g. wastewater) (Sheng et al 2010). EPS include mostly proteins, carbohydrates, humic substances (HS) and nucleic acids. The question of which microbial species participates most in mixed species biofilm matrix construction remains, in most cases, unclear. Anaerobic sludge granules are small spherical biofilms, which contain the different microbial groups typically found in anaerobic digestion consortia. Each group performs specialized metabolic functions sequentially leading to the transformation of the primary substrate into methane and C O2.
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