Abstract
Jumbo squid (Dosidicus gigas) is one of the largest cephalopods, and represents an important economic fishery in several regions of the Pacific Ocean, from southern California in the United States to southern Chile. Large and considerable discards of this species, such as skin, have been reported to constitute an important source of potential by-products. In this paper, a shotgun proteomics approach was applied for the first time to the characterization of the jumbo squid (Dosidicus gigas) skin proteome. A total of 1004 different peptides belonging to 219 different proteins were identified. The final proteome compilation was investigated by integrated in-silico studies, including gene ontology (GO) term enrichment, pathways, and networks studies. Potential new valuable bioactive peptides such as antimicrobial, bioactive collagen peptides, antihypertensive and antitumoral peptides were predicted to be present in the jumbo squid skin proteome. The integration of the global proteomics results and the bioinformatics analysis of the jumbo squid skin proteome show a comprehensive knowledge of this fishery discard and provide potential bioactive peptides of this marine by-product.
Highlights
Marine by-products are the body parts of marine species that are removed before they reach the final consumer in order to improve their preservation, reduce the shipping weight, and increase the quality of the main product [1,2]
A shotgun proteomics strategy was applied for the first time for the characterization of the jumbo squid skin proteome
The most prominent protein classes were oxidoreductases, calcium-binding proteins, hydrolases, nucleic acid binding, enzyme modulation, transferases involved in metabolic pathways, endocytosis/phagosome, RNA transport, protein methylation, and calcium homeostasis
Summary
Marine by-products are the body parts of marine species that are removed before they reach the final consumer in order to improve their preservation, reduce the shipping weight, and increase the quality of the main product [1,2] These organic materials are the main concern for current fishery management policies and legislation because they represent a significant source of valuable compounds such as proteins, minerals and lipids. From 2019 new regulations of fishery landing in the European Commission (EU) (European Commission Regulation (EU) No 1380/2013) oblige to keep and not discard all the species that are caught that are subjected to quota as well as underutilized commercial species [3] For this reason, valorization solutions of marine discards biomasses have to be implemented. It represents an important economic fishery resource in a wide number of countries such as Chile, Peru, Japan, and Mexico [8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
