Abstract
AbstractThe hydrophobic acid portion (WSE‐FA) was isolated from a water‐soluble extract of wheat (Triticum aestivum L.) straw and chemically characterized. This material was isolated and concentrated on a column containing Amberlite XAD‐8 resin. Chemical analysis of the WSE‐FA included elemental composition (C, O, H, N, S, P, and ash); titrametric evaluation of carboxylic acid and phenolic functional group content; molecular weight (MW) determination by vapor pressure osmometry (VPO); gel filtration and ultrafiltration; and UV‐visible, solid‐state cross‐polarization magic angle spinning 13C nuclear magnetic resonance (NMR), and Fourier‐transform infrared spectroscopy. The MW of the WSE‐FA was approximately 600 daltons. The 13C‐NMR spectrum, the low MW relative to other humic substances, high E4/E6 ratio, and the low N content suggested that the WSE‐FA was comprised of small, aromatic phenolic acids, oxidized polyphenols with exposed carboxyl groups, and saccharides that were condensed during senescence.
Published Version
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